Identification of miRNA differentially expressed in macrophages exposed to Porphyromonas gingivalis infection
Files
Main poster
Date
2016-04-10
DOI
Authors
Al-Hashemi, Jacob
Huck, Olivier
Drizik, Eduard
Gower, Adam
Davideau, Jean-Luc
Tenenbaum, Henri
Amar, Salomon
Version
OA Version
Citation
Abstract
We analyzed bacterial modulation of miRNAs in bone-marrow- derived macrophages (BMMs) induced by infection with either wild type Porphyromonas gingivalis (Pg) or mutant Pg (∆FimA), through a microarray analysis. TNF-α and IL-10 concentrations in Pg infected BMMs transfected with selected miRNAs were also assessed. The inhibition of mmu-miR-2137 increased the secretion of anti-inflammatory IL-10, while mmu-miR-155-5p decreased TNF-α. In vivo: injecting these miRNAs with Pg in mice reduced the size of the lesion significantly.