A progesterone biosensor derived from microbial screening
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Published version
Date
2020-03-09
Authors
Grazon, C.
Baer, R. Cooper
Kuzmanović, U.
Nguyen, T.
Chen, M.
Zamani, M.
Chern, Margaret
Aquino, P.
Zhang, X.
Lecommandoux, S.
Version
Published version
OA Version
Citation
C. Grazon, R.C. Baer, U. Kuzmanović, T. Nguyen, M. Chen, M. Zamani, M. Chern, P. Aquino, X. Zhang, S. Lecommandoux, A. Fan, M. Cabodi, C. Klapperich, M.W. Grinstaff, A.M. Dennis, J.E. Galagan. 2020. "A progesterone biosensor derived from microbial screening.." Nat Commun, Volume 11, Issue 1, pp. 1276 - ?. https://doi.org/10.1038/s41467-020-14942-5
Abstract
Bacteria are an enormous and largely untapped reservoir of biosensing proteins. We describe an approach to identify and isolate bacterial allosteric transcription factors (aTFs) that recognize a target analyte and to develop these TFs into biosensor devices. Our approach utilizes a combination of genomic screens and functional assays to identify and isolate biosensing TFs, and a quantum-dot Förster Resonance Energy Transfer (FRET) strategy for transducing analyte recognition into real-time quantitative measurements. We use this approach to identify a progesterone-sensing bacterial aTF and to develop this TF into an optical sensor for progesterone. The sensor detects progesterone in artificial urine with sufficient sensitivity and specificity for clinical use, while being compatible with an inexpensive and portable electronic reader for point-of-care applications. Our results provide proof-of-concept for a paradigm of microbially-derived biosensors adaptable to inexpensive, real-time sensor devices.
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© The Author(s) 2020. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.